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KMID : 0665219930060040307
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Korean Journal of Food and Nutrition
1993 Volume.6 No. 4 p.307 ~ p.313
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Purification and Some Physicochemical Study of Bacillus cereus β-Amylase
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Abstract
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Bacillus cereus B-amylase was purified by Sephadex G-100 gel filtration, CM Sephadex C-50 ion exchange chromatography and CM Sephadex C-50 ion exchange rechromatography. The purified enzyme showed 871unit/㎎ of specific activity. The purified enzyme was identified as homogenious by disc PAGE, SDS-PAGE and analysis of reaction product. The purified enzyme showed optimum pH 7.0. optimum temperature 50℃, and was stable at 0∼50℃ and at pH range of 6∼10.
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